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ISOLATE II Plasmid Mini Kit 10 Preps, Mini Kit plasmid, Plasmid DNA isolation, purification, extraction, preparation, transfection

Product Features:

    The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol.By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a…
Price as on: 2019-02-05 14:50:12
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Product Description

The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol.By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria.The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.

Product Features

  • Fast – streamlined protocol for extraction of eighteen plasmid samples in as little as 15 minutes
  • Efficient – rapid SDS/alkaline lysis and efficient column-binding for high-yield recovery of all plasmid molecules
  • High-performance – efficient extraction of high-purity plasmid DNA, ideal for use in all downstream applications
  • Flexible – reliable recovery of high- and low-copy plasmids from all gram-positive bacteria